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CHOFlpIn cells used for mammalian cell studies were maintained in DMEM supplemented with 5% FBS at 37C in 5% CO 2

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To investigate the effects of microplastics on neurodevelopment in human pluripotent stem cell-derived cortical spheroids, it was found that long-term exposure (4 to 30 days) led to decreased cell viability and downregulated the expression of mature neuronal markers, such as -tubulin III and the cortical layer VI markers TBR1/TBR2, indicating adverse effects of microplastic exposure on early human brain development

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