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Upon reaching 80% confluency, cells were washed with cold PBS and mechanically scraped into 1 mL of ice-cold Chelex-100-treated PBS containing 0.05% v/v benzonase (Sigma

The combination tends to produce better body composition outcomes than either alone

From this model, it is estimated that as a result of the competition with icodec for albumin binding locally at the injection site, less than 1% of semaglutide is bound to albumin in the subcutis following IcoSema administration (Alifrangis L., 2025, unpublished data)

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