Additives : Antioxidants or reducing agents (e.g., DTT) may help, but use cautiously as they can interfere with some assays
Journal of renal nutrition: the official journal of the Council on Renal Nutrition of the National Kidney Foundation
Separated proteins were transferred onto Immobilon-P Transfer membranes (MilliporeSigma, IPVH00010), blocked for 1 h using 5% BSA in TBS0.5% Tween-20 and stained overnight with primary rabbit-anti-GCLC (Sigma-Aldrich, HPA036359) or affinity-purified rabbit-anti-GGT1 (GGT129, as previously described 63 ) 1:1,000 in BSA 2.5% overnight at 4 C
Genetic Variations (GSTM1, GSTP1 Polymorphisms) Polymorphisms in genes encoding glutathione S-transferases (GSTs) can reduce detoxification capacity and glutathione efficiency by 20-40%
Several classes of GST have been shown to exhibit non-catalytic ligand binding in the dimer interface 13,15
Dermatologists generally recommend avoiding alcohol during the treatment period to allow glutathione to work effectively