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Q-PCR reaction mixtures contained 1 l cDNA, 1x SYBR Green Supermix (BioRad, Hercules, CA), and 0.15 M gene-specific primer sets in a total volume of 25 l
Structurally, MT-1 is long and linear, while MT-II has a circular amino acid arrangement
The purified recombinant protein of F0726_RS04210 showed remarkable GR activity at optimal pH 7.0 and 30C using in vitro assay
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Using preclinical models of breast cancer, the team also found they could slow tumor growth by blocking the cancer's ability to use glutathione
