Gels were homogenized with a polypropylene EPPI-pestle homogenizer in the eppendorf tube for 10 to 20s at room temperature until most of the homogenate pieces were small enough to pass through a 1 mL micropipette tip
A separate 50 l of reaction buffer containing various concentrations of p NPP (50, 100, 150, 200 or 250 mM) was also preincubated at 30 C for 1 min
Pearson-Smith and Patel, 2017)
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Sometimes, it's carried in specialty grocery stores or sold at farmer's markets
However, peptide therapy offers the advantage of extended treatment duration (daily dosing over weeks) versus the single or limited injection approach of PRP