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Visceral adipose tissue is itself a primary driver of insulin resistance
Another process is chelation, where GSH plays a role as a precursor of phytochelatins (PCs) synthesis
In the aforementioned study from our group, adult mice with partial IGF-1 also displayed decreased expression of genes involved in lipid metabolism (ATP-citrate lyase, acetyl-CoA acyltransferase 1B, acetyl-CoA acetyltransferase 1) and cholesterol synthesis and transport (Both HMG-CoA reductase and synthase, LDL-related protein 1, proprotein convertase subtilisin/Kesin type 9), resulting in dyslipidaemia
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Each sample was thereafter incubated with 10 l of acetonitrile and stored at 20 C before analysis was performed on a Beckman HPLC instrument operated with System Gold coupled to diode array UVVIS detector module 168
Equal amounts of cell protein lysates were separated by SDS PAGE and transferred to PVDF membranes (Millipore, Darmstadt, Germany)
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