Despite emerging therapeutic therapies that provide potentially effective treatment of NDs, a great deal has to be accomplished, and recent interest in preclinical studies and the first translations of some therapies into clinics has paved the way for further development in this direction
ROS originate from normal cell metabolic processes as well as exogenous sources like ultraviolet or ionizing radiation, and chemicals
Anisimov VN, Arutjunyan AV, Khavinson VK
Shimizu H, et al
When oxidative stress is left unchecked, it can lead to the formation of harmful substances that damage blood vessels, contributing to conditions like atherosclerosis and high blood pressure
WAT mitochondria isolation To isolate mitochondria, fresh gWAT was minced then homogenized using a drill-operated Teflon pestle in ice-cold MSHE buffer (70 mM sucrose, 210 mM mannitol, 5 mM HEPES, 1 mM EDTA, pH 7.2) containing 0.5% FA-free BSA