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At the end point, 2.5 M Bodipy 581/591 C11 (Cell Signaling, 95978) was added directly to each well and incubated for 30 min

That selectivity matters in research for two reasons: Cleaner interpretation: If a compound pushes fewer off-target endocrine pathways, observed outcomes are easier to connect to the intended mechanism

E., Bartels, T

This process is irreversible

Transsulfuration: Hcy combines with serine to form cystathionine, helped by vitamin B6, which breaks down into cysteine for making antioxidants like glutathione

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