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The cells were then washed three times with PBS containing 1% BSA (w/v) followed by 1 h incubation with 1:1,000 anti-mouse Alexa Fluor 488 conjugated secondary antibody (diluted with PBS containing 5% BSA, Invitrogen) at RT in the dark
Sigue siempre las indicaciones del fabricante y no superes la dosis recomendada
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It's also common to lose some muscle mass along with fat while on these drugs, which can be problematic, Kessler writes
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