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And it rewards patience

The maximum plasma concentration (C max ) reached for a standard dose of PZQ (1,500 mg), in healthy volunteers, is 0.16 g/ml or 513 nM 64

Under normal physiological conditions, small amounts of 5-oxoproline are derived from gamma-glutamyl-cysteine by the action of gamma-glutamyl cyclotransferase (63)

Procedure Preheat spectrophotometer/microplate reader for 30 minutes, adjust the wavelength to 412 nm, set zero with distilled Preheat Reagent II in 37 (mammal cell) or 25 (other species) water bath for 30 Blank tube determination: take micro glass cuvette, add 20 L of distilled water, 140 L of Reagent II, 40 L of Reagent III in turn, mix well, place for 2 minutes, and measure 412 nm absorbance AB

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Journal of Pakistan Association of Dermatologists