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Vitrified sample preparation and data collection Samples (3L) were applied to glow-discharged Quantifoil R1.2/1.3 Cu/Rh 200 mesh grids (Quantifoil GmbH, Grolbichau, Germany) and were flash frozen in liquid ethane using a Vitrobot mark IV (Thermo Fisher Scientific, Waltham, Massachusetts, USA) set at 100% humidity and 4C for the prep chamber and 10 s blot time

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