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U2OS cells stably expressing G3BP1-GFP or U2OS cells in which TIA1, caprin 1, TDP43, or G3BP1 had either GFP or mRuby3 stably encoded into their endogenous C-terminal loci using CRISPR-Cas9 were cultured in DMEM (Invitrogen, 11995-065) supplemented with 10% FBS, penicillin/streptomycin (Invitrogen), and GlutaMAX (Invitrogen) at 37C (5% CO 2 )
This is why we focus so heavily on liposomal technology, as explained in our All About Liposomes educational page
K-SC and D-HK designed paper and supervised the manuscript
Your Move: Actions For the Public: Share clinical trial experiences responsibly
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