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Research ArticleGastroenterologyMicrobiology Free access | 10.1172/jci.insight.132055 1 Department of Internal Medicine-Molecular Medicine, Wake Forest School of Medicine, Winston-Salem, North Carolina, USA

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To explore the interactions between AvrPto and neighboring immune system proteins, the authors designed four constructs: AvrPto_BirA (fusion of AvrPto with the N-terminus of BirA), MP_BirA (fusion of an 8-amino acid AvrPto myristoylation/palmitoylation (MP) motif with the N-terminus of BirA), BirA alone, and an empty vector (EV) control

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