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These markers correlate directly with pineal gland melatonin output and indicate whether circadian-targeted therapy is physiologically indicated

2019 Aug 8;9(8):e025884

Determination of serum transaminases Serum levels of hepatic function markers alanine aminotransferase (ALT), aspartate aminotransferase (AST), and creatinine were determined using commercial high-quality optimized Elabscience ELISA test kits (MD, USA) in a microplate reader, according to the manufacturers instructions (Zhao et al

Roche, Basel, Switzerland), and hybridization was carried out overnight at 68 C

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