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Two days after siRNA transfection, cells were transfected with an additional 0.5 l PDS5B siRNA (jkSIR012: 20 M), 1.2 g HA-TwinStrepTagII-PDS5B wild type or the 17 A mutant, 0.8 g I-SceI expression vector (pCBASce), and 5 l Lipofectamine 2000
A total of 45 trials were included in the meta-analysis
Clinical Therapeutics
A useful note records the numeric value, the unit, the laboratory range, and the date, along with why the test was ordered and whether the elevation was incidental or clinically prompted
We further verified the enhanced levels of H1(N76D/N77D) around DSBs using a U2OS-AsiSI-ER-AID cell line, in which site-specific DSBs can be induced by 4-hydroxytamoxifen (4OHT)
autoantibody levels are usually high
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