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The above-reported evidence suggests that, on the basis of the initial localization of pathological forms of -syn, different subtypes of PD can be identified in addition to the traditional staging framework based on the canonical Braak pattern, which is characterized by the caudo-rostral spread of -syn-positive LBs, which originate in the brainstem and systematically ascends toward the neocortex [101, 102]

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The chromatographic separation was performed on an Agilent 1290 Infinity UHPLC-system (Agilent Technologies, Waldbronn, Germany)