I've just described myself sprinting through the airport and going, "All right, Andy Galpin, I got my 90 seconds max heart rate in for you, carrying this thing." But 20 seconds on, 10 seconds off, repeating that over and over for 10 minutes
For the steady state, 50 l measurement buffer was added to 450 l cell suspension and fluorescence was measured for 2 min
Perry EK, Lee MLW, Martin-Ruiz CM, Court JA, Volsen SG, Merrit J, Folly E, Iversen PE, Bauman ML, Perry RH, Wenk GL (2001) Cholinergic activity in autism: abnormalities in the cerebral cortex and basal forebrain
Methionine adenosyltransferase 1 is targeted to the mitochondrial matrix and interacts with cytochrome P450 2E1 to lower its expression
Enzymatic activity assay A solution of the TPE-DCV and GSSG mixture (2.0 mL) was placed in a quartz cuvette (final concentration: [TPE-DCV] = 3 M, [GSSG] = 1 mM)
Meister A, Anderson ME