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Melanoma cells then have the capacity to undergo clonal expansion to invade and destroy local tissues, to metastasize to regional lymph nodes, and to spread to the lungs, bone, liver, brain, or skin [15, 5254]
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these correspond to binding of the secondary antibody to the light and heavy chains of the anti-tubulin antibody originally used to capture tubulin from the lysate
Stahl, "Inhibition of Glutamine Synthetase in A549 Cells During Hyperoxia", Am