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After 5 min, 10 L spread solution (200 mM Tris-HCl, pH 7.5, 50 mM EDTA, and 0.5% SDS) was spotted on the cell droplet and spread on slides

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Important Note: Seek urgent medical care for trouble breathing, swelling of the face or throat, widespread hives, dizziness, or symptoms that suggest a serious allergic reaction

The products were then separated via SDSpolyacrylamide gel electrophoresis, transferred onto nitrocellulose membranes, and blotted with an anti-TRPV1 antibody (#ACC030

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Storage is another consideration that Canadian researchers sometimes overlook