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A.WangG
At the desired time point, cells were fixed with 4% PFA for 45 min, permeabilized with 0.2% Triton X-100 (Sigma, 11332481001) diluted in phosphate-buffered saline (PBS) for 10 min and blocked with 1% bovine serum albumin (Sigma, cat
Then, they feel a strong heat all over, causing them to sweat and look for a cooler place
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M.DewachterI
6c) of control PBS-treated DMM samples revealed the severe destruction of articular cartilage at the tibial plateau (highlighted by red arrows) and exposure of the subchondral bone, thus validating the OA model
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