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Notably, we generated various N-terminal truncation constructs for SnCE1, i.e., SnCE1 74-310 , SnCE1 85-310 , SnCE1 95-310 and SnCE1 104-310 , the catalytically inactive mutant SnCE1 C256A and the reported gatekeeper mutant SnCE1 W191A (Fig