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PMID: 31305971 [18] Lisco G, De Tullio A, Disoteo O, et al

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inspect solution before each use (should be clear and colorless) [2]

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Before every injection, hold the syringe needle-up, tap the barrel to move bubbles to the top, and push the plunger gently until a tiny droplet appears at the needle tip

Upon reaching the isoelectric point, various physical properties of ampholytic electrolytes, such as solubility, viscosity, and osmotic pressure, minimize, a phenomenon particularly evident in protein solutions