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However, by using different FLNA dominant negative mutants that selectively abolished the ability of FLNA to interact with SST2 (FLNA repeats 1920) or to function as scaffold for partner proteins (FLNA 2124), the authors demonstrated that FLNA plays both a structural role, by stabilizing SST2 expression after prolonged stimulation with a specific agonist, and a functional role, acting as scaffold protein for molecules involved in signal transduction

After washing and resuspension, the sample tubes were analyzed by flow cytometry by BD FACSymphony

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