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These include Alzheimers Disease where neuroprotective effects are under investigation followed by stroke under similar notion (125) Futhermore, in non-alcoholic fatty liver disease (NAFLD) they show evidence of improved liver histology and hepatic steatosis (126) in PCOS they may help in regulation of weight, reproductive and metabolic parameters simultaneously (127) GLP1-Ras are also under study with their ability to modulate the hemodynamic and inflammatory pathways to treat CKD (128) and Heart failure (129)

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The standard procedure and reagents from the Q5 Site-Directed Mutagenesis kit (NEB) were used to insert mutations, ligate plasmids and transform them into NEB 5 competent cells by heat shock