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RNA quality was assessed by Agilent 2100 Bioanalyzer using the RNA 6000 Nano Chip (Agilent Technologies, Amstelveen, The Netherlands), and RNA quantification was performed using ND-2000 Spectrophotometer (Thermo Inc., DE, USA)

Naturally present in plasma, saliva, and urine, its levels decline with age, correlating with slower healing and visible tissue degeneration

After four rinses in 0.1% BSA-C in PBS, the samples were incubated with a 10-nm gold-conjugated secondary goat anti-rabbit IgG antibody (Amersham International, Little Chalfont, United Kingdom) diluted 1:50 in 0.1% BSA-C in PBS for 4 h at 37C

Rhein attenuates cerebral ischemia-reperfusion injury via inhibition of ferroptosis through NRF2/SLC7A11/GPX4 pathway

Administration should occur only under the supervision of a licensed healthcare professional with appropriate patient evaluation, documentation, and informed consent

In: Vlamos P (ed) GeNeDis Advances in Expt Med Bio