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Stage-3 Synthesis of Semaglutide by solution phase peptide fragment condensation: Step-A: Gln(Trt)-Ala-Ala-Lys(C18diacid mono-t -butyl -y-Glu(AEEA-AEEA)-OtBu)-Glu(OtBu) -Phe-Ile -Ala-Trp(Boc)-Leu-Val-Arg(Pbf)-Gly-Arg(Pbf)-Gly-OtBu (3.7 grams) (fragment-6) was dissolved in DMF and stirred for 10 minutes at 25-30 C
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Meanwhile, heat a drizzle of oil in a second large pan over medium-high heat
