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Cell 168 , 960976 (2017)
Pre-Clinical Studies Most previous preclinical studies have used transgenic mice overexpressing human mutant SOD1
Whyte WL et al
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Since D2HG and L2HG are indistinguishable in standard LC-MS, to determine which 2HG enantiomer(s) is affected, we adapted a chiral N-(p-toluenesulfonyl)-L-phenylalanyl chloride (TSPC) derivation followed by liquid chromatography with tandem mass spectrometry (LC-MS/MS) analysis 24,40
All assessments were conducted by an experienced technician under blinded conditions
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