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Nanoflow LC-MS/MS was conducted using an Orbitrap Elite hybrid mass spectrometer (Thermo Fisher Scientific, Waltham, MA) with a nanospray ionization source, coupled to an Ultimate RSLCnano LC System (Thermo Fisher Dionex, Sunnyvale, CA)
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We separated the growth media from cells grown at a fixed temperature by flowing the liquid cultures through 0.45m-pore filters as previously described
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