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Key takeaways There is no quality scientific evidence to suggest that coffee enemas provide health benefits

Recreational nitrous oxide (laughing gas) misuse is an increasingly common cause of B12-related nerve damage in the UK and requires urgent assessment if neurological signs develop

Serin, Y

Repeated freeze-thaw cycling of a reconstituted preparation promotes aggregation, so aliquoting once and thawing each aliquot only once is standard practice

We do not have enough data to make strong claims either way

The consequences are significant: accelerated muscle loss, a soft flabby appearance despite weight loss, reduced metabolic rate, weakened immune function, hair loss, and poor recovery from exercise