At 48 hpi, the cells were fixed, permeabilised, and immunolabelled with the appropriate antibodies, and then processed for immunofluorescence microscopy
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Endothelial tube-formation and aortic-ring angiogenesis assays with VEGF/eNOS readouts
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A major concern which will need to addressed, is the high rates of discontinuation up to 50% - observed in recent studies
Morning injection aligns with natural circadian collagen synthesis rhythms