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Keep lyophilized vials cold until use, then reconstitute with sterile diluent under aseptic technique immediately before an experiment

In individuals with OA, the iron content in synovial fluid is elevated, and greater serum ferritin levels correlate with increased radiographic severity of the joints compared to healthy controls [86,87]

The lysates were subjected to electrophoresis, separated on 812% polyacrylamide gels and then transferred to PVDF membranes