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For example, a comprehensive analysis of TCGA-BRCA data showed recurrent mutations/variations were detected in genes such as TP53, PIK3CA, GATA3 , and MAP3K1 , associated with tumor initiation, progression, and therapy resistance (276)

Samples were fixed in 10% buffered formalin overnight at 4C, dehydrated with increasing concentrations of ethanol, embedded in paraffin, cut into 5 m sections, and stained with hematoxylin and eosin (HE) or Massons Trichrome Stain Kit (Sigma-Aldrich)

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The co-primary endpoints were the percentage change in body weight and achievement of weight loss of 5% at week 104