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GSH synthesis using the whole-cell biocatalyst was performed in 0.2 M phosphate buffer (pH 7.0) containing 40 mM l-glutamate, 20 mM l-cysteine, 40 mM glycine, 20 mM MgCl 2 and 20 mM ATP

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Given the reciprocal relationship between AMPK and mTOR signalling 36 and the links between mTOR and PGC1 signalling 37 , we next explored the role of mTOR