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To release the AAV9 virus, cell pellets were resuspended with AAV lysis buffer (50 mM Tris-HCl, pH 8.0, 150 mM NaCl), followed by three freeze-thaw cycles
This process involved metabolic dysfunction that results in increased production of cytosolic and lipid ROS, independently of mitochondria
This last step is often missing
Oncol Transl Med
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