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Any animal-model protocol should include defined humane endpoints, veterinary oversight, and IACUC or equivalent ethics-committee approval before initiation
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Interestingly, the eccDNA Replicon was recently found to have incorporated the coding sequence for Glutamine Synthetase 2 (GS2) into its amplicon, conferring glufosinate resistance and further supporting the idea of recombination and internal crosstalk among eccDNAs within the circulome ( 2.2 Functional and adaptive roles of eccDNA The properties outlined above position eccDNAs as independent, functional genetic units more analogous to bacterial plasmids than chromosomes that can increase genomic and phenotypic flexibility without altering the chromosomal DNA
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