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To evaluate whether GLP-1R and PKA signalling participated in the protective effect of metformin in HUVECs, siRNAs as well as pharmacological blocking were introduced in our study

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Compared with saline, DREADD-based activation of LS GLP-1R neurons via CNO reduced a 2-hour consumption period of both standard chow and high-sucrose food during the dark period when mice normally consume food actively (Figure 5, C and D)