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Consulting verified scientific literature indexed in open research resources like the NCBI PubMed database provides additional guidance regarding chemical stability profiles under varied laboratory temperatures and exposure windows
Functional tests can provide additional context Directly measuring vitamin and mineral levels in the blood isn't always enough
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Other assays Various in vitro cellular functional assays, gene/protein detections, and biochemical assays were performed as previously described in detail [23, 24], including assessments of cell proliferation (EdU-nuclear staining), migration (Transwell assay), invasion (Matrigel Transwell assay), capillary tube formation, reactive oxygen species (ROS) production (through MitoSOX staining), mitochondrial depolarization (JC-1 staining), Caspase-3 and Caspase-9 activity, TUNEL-nuclear staining, mitochondrial Complex I activity, and cellular ATP levels
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