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En mamferos el ADN es metilado principalmente en residuos de citosina presentes en dinucletidos citosina-guanosina (CpG), a travs de una reaccin que transfiere un grupo metilo desde el dador S-adenosil-L-metionina a la posicin 5 del anillo de citosina, generando 5-metilcitosina11

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