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CTK solution (0.25% trypsin + 1 mg/mL collagenase IV + 1 mM CaCl 2 + 20% KSR in DPBS ()) was prepared with trypsin ((2.5%), no phenol red, Thermo Fisher Scientific Inc.), collagenase (type IV, powder, Thermo Fisher Scientific Inc.), calcium chloride dihydrate (Fujifilm Wako Pure Chemical Corporation), Knock Out TM Serum Replacement (KSR, Thermo Fisher Scientific Inc.)

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Studies reported that GLP1-RAs improve metabolic and reproductive parameters (such as menstrual regularity) in women with polycystic ovarian syndrome (PCOS), raising the possibility that GLP1-RAs may improve fertility in women with PCOS

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Lumps and swelling during the weeks following treatment are to be expected and will resolve on their own, given time and gentle treatment