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Mice were anesthetized, decapitated, and brains were removed and quickly immersed in cold (4 C) oxygenated cutting solution containing (in mM): 50 sucrose, 2.5 KCl, 0.625 CaCl 2 , 1.2 MgCl 2 , 1.25 NaH2PO 4 , 25 NaHCO 3 , and 2.5 glucose

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By suppressing ACC, AOD9604 reduces lipogenesis the formation of new fat deposits