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cDNA was prepared from total RNA and used for gene expression by real-time quantitative RTPCR (qPCR) as described [53] using TaqMan Gene Expression Assays (ThermoFischer Scientific) specific for human GCLC (Hs00155249_m1), OCT4 (Hs00999632_g1), and ACTB (Hs01060665_g1)
Both start as sterile water
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Throughout the post-harvest period, mushrooms undergo a sequence of quality deteriorations, including discoloration, moisture reduction, taste degradation, texture modifications, and nutrient depletion (8)
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