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Expression of a constitutively activated form of protein kinase B (c-Akt) in 3T3-L1 preadipose cells causes spontaneous differentiation

The outer thigh, midway between the hip and the knee, is a reliable subcutaneous injection site and is the preferred alternative when the abdomen is uncomfortable or if site rotation requires a break from abdominal injections

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Endotoxin levels should remain below 1 EU/mg for injectable use

These injections are typically administered by healthcare professionals to treat vitamin B12 deficiency in people with pernicious anemia and other conditions, but some clinics offer training for safe self-injection at home