Over time, due to stress, pollution, and a poor diet, there is a depletion of glutathione in the body, leading to a buildup of toxins and cellular damage
G., Slusher, B
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The cells were then centrifuged at 10,000 g for 2 min and incubated with 1 ml of 2 M HCl and 0.5% Triton X-100 (Sigma-Aldrich, 93443) for 30 min with gentle agitation
Toxicol Appl Pharmacol 437:115892
(ii) GSH exerts a concentration-dependent effect on NF-YA1 gene expression, which was downregulated in (h)GSH-depleted wild-type and sym33-3 roots with nodules and upregulated in both genotypes following treatment with the low (0.1 mM), but not high (1 mM) concentration of GSH