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Blood samples were collected at 0, 0.25, 0.5, 1, 2, 4, 6, and 15 hours after alcohol consumption to measure both alcohol and acetaldehyde levels using validated assay kits, capturing key pharmacokinetic parameters such as maximum serum concentration (Cmax) and area under the curve (AUC)

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Full-length GST-TAK1 (aa 1-606) and the N-terminus (aa 1-305) but not the GST-vector interacted with GPX4, suggesting that these two proteins directly interacted with each other (Fig

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