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Escherichia coli were harvested by centrifugation and resuspended in HEPES buffer (20 mM HEPES, 300 mM NaCl, pH 7.4)

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The more the content of glutathione , faster and more effective the treatment is

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In addition to a series of risks during and after the joint replacement operation, total knee replacement surgery occurs in as many as 5% of cases, requiring revision surgery ten years after the operation [4]