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Thyroid hormones exhibit profound metabolic effects characterized by an increased rate of both catabolic and anabolic reactions, resulting in an overall acceleration of the basal metabolism which is associated with increased oxygen consumption, respiratory rate, energy expenditure, and heat production (56)

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This process generates resistance to the anorexigenic hormones leptin and insulin, contributing to the genesis of obesity

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In this chapter, we describe the GST pull-down approach to detect the interaction between PIF4 and a known or suspected interacting protein

How is purity verified?+ Each production lot is subjected to quantitative HPLC (99% purity) and highresolution mass spectrometry for identity confirmation