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Plasmids of wildtype and mutants of ARE motifs were transfected to K562 (#CRL-3344, ATCC), an erythroleukemia cell, along with pDsRed1-N1 (PT3405, Clontech), which is used as a transfection efficacy control
in an additional analysis, we adjusted the hazard ratio for calendar year in three year intervals in a multivariable model (in addition to propensity score weighting)
Exact matching of treated and control subjects on the propensity score balances all observed covariates
Combination protocols give both an immediate GH pulse (from Ipamorelin) and a slightly extended pulse (from mod CJC), though still shorter than the DAC versions multi-day effect
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