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Cell and tissue lysates were sonicated for 30 s

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Optional research biomarkers: senescence markers (p16^INK4a, SA--gal), SASP cytokine profile (IL-6, IL-1, MMPs) if lab resources permit

The process stays the same, just repeated

It becomes a bit of a chicken and egg situation while Lyme and mycotoxins can cause MCAS, MCAS may prevent an individual from being able to treat the other diseases adequately because of major hypersensitivities it can create to the things that address them

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