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and a discussion with the patient regarding the potential need for rapid sequence induction (RSI) to minimize aspiration risk, versus postponement of the procedure

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Unlike the C-terminal amide of Melanotan II, the free carboxylate here is titratable and carries an additional negative charge at neutral pH, so record buffer pH alongside concentration when comparing the two molecules in the same assay

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*Please note that the linearity of the calibration curve will not be obtained if the coloration time is prolonged to enhance the coloration