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Data availability All data analyzed in this publication were publicly available, including the human lung MSI and spotted mouse liver from METASPACE ( and the murine urinary bladder from the Proteomics Identifications Database (PXD001283) and the 10X Visium and MALDI (FMP-10 and DHB matrices) Parkinson mouse brain data ( The IDH -mutant tumor data are available from the BioImage Archive ( All data objects used for vignettes can also be found on the SpaMTP Zenodo community page ( Databases used for metabolite annotation include HMDB ( released November 2, 2021), ChEBI ( released September 1, 2023), LIPIDMAPS ( released September 15, 2023) and GNPS ( released April 21, 2025)